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Fig. 4 | AAPS Open

Fig. 4

From: Nano-formulations composed of cell membrane-specific cellular lipid extracts derived from target cells: physicochemical characterization and in vitro evaluation using cellular models of breast carcinoma

Fig. 4

Effect of cholesterol and PEG inclusion on cellular uptake of CLENs. Four different preparations of 4 T1 CLENs containing 25 mol% of cholesterol and different mol% of DPPE-PEG-5000 (0, 2, 5 and 10%) were evaluated for cellular uptake against 4 T1 cell line. Rhodamine-labeled CLENs were used for this study. The cells were seeded in a 48-well plate followed by the addition of CLENs for 24 h. The fluorescence intensity used here as an indicator of cell uptake was measured using a fluorescence microplate reader. CLENs containing 5 mol% of DPPE-PEG-5000 demonstrated the most significant uptake compared to other varieties within the concentration range employed. Data are presented as mean ± S.D.; (n = 6)

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