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Table 1 Modified chart of the Dyer and Bligh lipid extraction method

From: Nano-formulations composed of cell membrane-specific cellular lipid extracts derived from target cells: physicochemical characterization and in vitro evaluation using cellular models of breast carcinoma

Cell Number (millions)

(5–9.9)

(10–24.9)

(25–49.9)

(50–74.9)

(75–99.9)

1:2 CHCl3: MeOH

0.750

1.9

3.75

5.7

7.5

CHCl3

0.250

0.625

1.25

1.875

2.5

dH2O

0.250

0.625

1.25

1.875

2.5

Total Volume

1.45

3.65

7.25

10.95

14.50