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Fig. 10 | AAPS Open

Fig. 10

From: Antibody-drug conjugates: integrated bioanalytical and biodisposition assessments in lead optimization and selection

Fig. 10

Quantitative mass spectrometry based assay (LC-MRM) of intact Pep-ADC. This assay measures the concentration of the intact Pep-ADC by monitoring peptide R1–12 which covers the first 12 amino-acids of Pep-ADC (a). The relative concentration (compared to immediately after dosing) of intact Pep-ADC and main metabolite (M1) in plasma samples from C57BL/6 J normal mice IV dosed with 3 mg/kg of Pep-ADC (b). Data plotted are mean from three mice per time point (Exception: Day 3 has n = 2). Error bars correspond to the S.D. calculated using GraphPad Prism 7. Error bars shorter than the symbols are not plotted

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