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Table 2 Experimental conditions for the universal HPLC method

From: Non-isothermal stability by linear heating: a fast method for preformulation stability screening of drugs at the discovery and development interface

HPLC System Waters E-2695 Module
Detector Waters 2489 UV detector and 2998 PDA detector
Software Waters Empower 2
Column Agilent Zorbax SB-C18 3.5μm 4.6x150mm
Mobile Phase A: 0.05% TFA in DI Water
B: Acetonitrile
Gradient Time (min) A (%) B (%)
0 80 20
2 80 20
20 10 90
25 10 90
26 80 20
28 80 20
Flow Rate 1 mL/min
Injection Volume 20 μL
Running Time 28 min
Column Temperature 30 °C
Wavelength Selected based on each compound’s λmax