Non-isothermal stability by linear heating: a fast method for preformulation stability screening of drugs at the discovery and development interface

AAPS Open

Table 2 Experimental conditions for the universal HPLC method

HPLC System	Waters E-2695 Module
Detector	Waters 2489 UV detector and 2998 PDA detector
Software	Waters Empower 2
Column	Agilent Zorbax SB-C18 3.5μm 4.6x150mm
Mobile Phase	A: 0.05% TFA in DI Water
Mobile Phase	B: Acetonitrile
Gradient	Time (min)	A (%)	B (%)
	0	80	20
	2	80	20
	20	10	90
	25	10	90
	26	80	20
	28	80	20
Flow Rate	1 mL/min
Injection Volume	20 μL
Running Time	28 min
Column Temperature	30 °C
Wavelength	Selected based on each compound’s λ_max