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Fig. 2 | AAPS Open

Fig. 2

From: Antibody-drug conjugates: integrated bioanalytical and biodisposition assessments in lead optimization and selection

Fig. 2

In vitro stability of anti-CD25-Phos-Dex and anti-CD25-Cat-Dex in mouse plasma. To assess the trend in stability of these antibodies, Anti-CD25-Phos-Dex (a) or anti-CD25-Cat-Dex (b) was spiked in normal male DBA1 mouse plasma for up to 18 days at 37 °C. After incubation, the levels of antibody was determined by total antibody immunoassay (Total IA) and the levels of intact ADC was determined by ADC specific immunoassay (ADC IA) on plasma samples using Mesoscale Discovery (MSD) sandwich immunoassay, and free dexamethasone was determined by LC-MS (Free Dex). Amounts in picomole (pmol) versus time are plotted to compare across different methods of analysis. Values represent the results of a single incubation at each time point. Panel c shows the unexpected site of cleavage in the cathepsin linker of anti-CD25-Cat-Dex

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