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Fig. 5 | AAPS Open

Fig. 5

From: Antibody-drug conjugates: integrated bioanalytical and biodisposition assessments in lead optimization and selection

Fig. 5

In vitro stability of unconjugated anti-CD74 (a), anti-CD74-Phos-Bud (b), and isotype matched control antibody, mAb-C (c). Shown are the fluorescence SEC-HPLC profiles of DyLight 650-labeled antibodies in undiluted C57BL/6 J mouse plasma following incubation at 37 °C for 5 min, 7 days, and 14 days. The plasma samples were run on a BioSep-SEC-S3000 column at 1 mL/min elution rate with PBS as a mobile phase for 15 min. Black arrows indicate the percent of intact, monomeric IgG at day 14

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