Fig. 9From: Antibody-drug conjugates: integrated bioanalytical and biodisposition assessments in lead optimization and selectionIntact mass analysis of Pep-ADC in mice by AP-LC-MS. Pep-ADC (3Â mg/kg) administered to C57BL/6Â J diet induced obese (DIO) mice by IV was retrieved by pull-down for intact mass analysis on a Synapt G2S Q-TOF. Intact mass profiles show the appearance of several metabolites named M1, M2, and M4 (Panel a). Panel b shows the relative abundance of each species up to 10Â days post-dosing. Data plotted are mean peak intensity from four mice per time point. Error bars correspond to the S.D. calculated using GraphPad Prism 7. Error bars shorter than the symbols are not plotted. Panel c indicates the sites of cleavages on the peptide that matches with metabolitesBack to article page