Skip to main content

Pharmacokinetics and biodistribution of a novel anticancer thyrointegrin αvβ3 antagonist: triazole modified tetraiodothyroacetic acid conjugated to polyethylene glycol (P-bi-TAT)

Abstract

We previously developed a triazole modified tetraiodothyroacetic acid (TAT) conjugated to a polyethylene glycol (PEG)-based thyrointegrin αvβ3 antagonist targeted compound, called P-bi-TAT. It exhibited potent anti-angiogenic and anticancer activities in vivo. The objective of the current study is to develop a quantitative bioanalytical method for P-bi-TAT using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and to elucidate pharmacokinetics (PK) and biodistribution of P-bi-TAT in animals. We used in-source collision-induced dissociation (CID) for ionization of P-bi-TAT in the positive mode, followed by multiple reaction monitoring (MRM) for quantification. P-bi-TAT was quantified using P-mono-TAT as an internal standard because of its similarity in structure and physicochemical properties to P-bi-TAT. The LOQ for P-bi-TAT was 30 ng/μL and the recovery efficiency was 76% with the developed method. Cmax and AUC results at different doses (1, 3, 10 mg/kg) in rats suggest that P-bi-TAT is dose-dependent within the range administered. Results for Cmax and AUC in monkeys at a low dose (25 mg/kg) were comparable to those in rats. Biodistribution of subcutaneously administered P-bi-TAT in the brain of rats ranged from 7.90 to 88.7 ng/g brain weight, and levels of P-bi-TAT in the brain were dose-dependent. The results suggest that P-bi-TAT is a potential candidate as a molecular-targeted anticancer therapeutic with blood-brain barrier permeability and acceptable PK parameters. Its accumulation in organs, toxicokinetic, and pharmacodynamics needs to be further investigated.

Graphical Abstract

Introduction

Cancer was the second leading cause of death in the USA in 2018, after heart disease (Xu et al. 2020). It is not a single disease but a variety of different cancer types including myeloma, thyroid, leukemia, esophagus, non-Hodgkin lymphoma, stomach, and prostate cancer (Henley et al. 2020). Among cancers, brain cancer is difficult to cure because of its poor prognosis, and the blood-brain barrier and blood tumor barrier that restrict the delivery of therapeutics (Fox et al. 2019). There are FDA-approved anticancer agents against brain cancers such as temozolomide; however, their efficacies are not enough to stop brain tumor progression beside their significant adverse effects (Anthony et al. 2019). Thus, more efficient, and safer anticancer therapeutics and improved drug delivery systems are under investigation in the pharmaceutical industry and in academia.

Targeted anticancer drugs have been developed and applied to multiple cancer types, such as glioblastoma and neuroblastoma (Laquintana et al. 2009; Le Rhun et al. 2019; Maeda et al. 2016). Among them, nanoparticle-conjugated compounds show selective efficacy and potency to tumors via the enhanced permeability retention (EPR) effect (Maeda et al. 2016). We previously synthesized a conjugate of tetraiodothyroacetic acid (tetrac) and poly (lactic-co-glycolic acid) (PLGA) polymer, PLGA-diamino-propane-tetraiodothyroacetic acid (DAT), and nanoparticles of DAT (NDAT) showed high potency and efficacy on multiple cancers, such as glioblastoma and neuroblastoma (Lin et al. 2016; Lin et al. 2018; Sudha et al. 2017a). We then developed a conjugate of β-cyclodextrin with triazole-modified-tetraiodothyroacetic acid (TAT), named β-C-TAT (CTAT). It showed anti-angiogenic in a chicken embryo chorioallantoic membrane (CAM) assay and anticancer activities in human primary GBM cells and in glioblastoma xenografted mice (unpublished data). Encouraged by the results, we then synthesized a polyethylene glycol (PEG)-based targeted drug, P-bi-TAT (1, Fig. 1), which is a conjugate of PEG4000 with two TAT moieties (Sudha et al. 2017b; Rajabi et al. 2019). It showed high affinity with a receptor on integrin αvβ3 (IC50 0.14 nM), anti-angiogenic activity in a CAM assay, and anticancer activities in human primary GBM cells and in glioblastoma xenografted mice (10 mg/kg) (Rajabi et al. 2019). In the current work, we developed a bioanalytical method for P-bi-TAT to determine its pharmacokinetics (PK) and biodistribution in experimental animals, i.e., rats and monkeys.

Fig. 1
figure1

Chemical structures of P-bi-TAT (1) and P-mono-TAT (2); and n means the average number of repeating oxyethylene units (90)

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a gold standard for quantification of drug molecules. However, typical quadrupole-type mass spectrometers are not applicable for P-bi-TAT because its molecular weight is more than 3000, and the quantification of high molecular weight PEGylated drug conjugates is a challenge by mass spectrometry. Yin et al. reported a quantitative method for PEGylated gemcitabine (Mw 70-76 kDa) using LC-time of flight mass spectrometer (TOFMS) coupled with pre-column derivatization and MSALL technique and applied to PK study of PEGylated gemcitabine in rats (Yin et al. 2020). In this study, we used in-source collision-induced dissociation (CID) for ionization of P-bi-TAT, followed by multiple reaction monitoring (MRM) (Warrack et al. 2013). P-mono-TAT (2, Fig. 1), which is another conjugate of PEG4000 with one TAT moiety, was used as an internal standard (IS) because its structure and physicochemical properties are similar to P-bi-TAT. We also optimized the extraction procedure from biological matrices using solid-phase extraction (SPE) with modification of the developed method for CTAT.

Experimental

Materials

Chemicals and biologicals

P-bi-TAT and P-mono-TAT were synthesized and purified in our laboratory. Acetonitrile, methanol, water, and formic acid were LC-MS grade and purchased from Sigma-Aldrich (St. Louis, MO). o-Phosphoric acid (85%) was HPLC grade and purchased from Fisher Scientific (Pittsburgh, PA). Rat and monkey plasma were purchased from Bioreclamation IVT (New York, NY). Rats were purchased from Charles River (Kingston, NY).

Methods

P-bi-TAT-treatment of rats and monkeys

P-bi-TAT-treatment of rats was carried out in the animal facility of the Veterans Affairs (VA) Medical Center (Albany, NY), and the experimental protocol was approved by the Institutional Animal Care and Use Committee of the VA. Rats were housed under controlled conditions (temperature, 20–24 °C; humidity, 60–70%). Rats were allowed to acclimatize for at least 1 week prior to the start of treatments. For the PK study, P-bi-TAT (10 mg/kg body weight) was administered subcutaneously to 4 rats, and plasma was collected at 0.25, 0.5, 1.5, 3, 6, 12, 24, and 48 h. Plasma samples were stored at −80 °C for further analysis. For the biodistribution study, P-bi-TAT (1, 3, 10 mg/kg) was administered subcutaneously to 4 rats once a day for 3 weeks, and brain samples were collected 24 h after the last dose. Brain samples were stored at −80 °C for further analysis.

The monkey study was conducted by a contract research organization. Briefly, P-bi-TAT (low dose, 25 mg/kg body weight; high dose, 82.5 mg/kg) was administered subcutaneously to male and female cynomolgus monkeys (n = 2 each) daily for 7 days, and plasma was collected at 0.5, 2, 6, 10, 16, and 24 h on day 1 and day 7. Plasma samples were stored at −80 °C for further analysis.

Optimization of solid-phase extraction for sample preparation of P-bi-TAT in plasma

Fifty microliters of rat and monkey plasma with or without P-bi-TAT (150 ng/μL) and IS (P-mono-TAT, 150 ng/μL) was vortex-mixed with 100 μL 4% H3PO4 and loaded on a C18 or an OASIS HLB SPE column (Waters, Milford, MA) in triplicate, followed by washing with 1 mL of 5% methanol in water. P-bi-TAT and IS were eluted with 1 mL of acetonitrile, acetonitrile/water (80/20 v/v), acetonitrile/water (50/50 v/v), and acetonitrile/water (20/80 v/v). After centrifugation, solvent was evaporated under a nitrogen stream at 50 °C. Extracts were reconstituted with 50 μL of acetonitrile/water (80/20 v/v).

Sample preparation of plasma with solid-phase extraction

Fifty microliters of rat and monkey plasma with or without P-bi-TAT (150 ng/μL) and IS (P-mono-TAT, 150 ng/μL) was vortex-mixed with 100 μL 4% H3PO4 and loaded on an OASIS HLB SPE column (Waters, Milford, MA), followed by washing with 1 mL of 5% methanol in water. P-bi-TAT and IS were eluted with 1 mL of acetonitrile/water (80/20 v/v). After centrifugation, solvent was evaporated under a nitrogen stream at 50 °C. Extracts were reconstituted with 50 μL of acetonitrile/water (80/20 v/v).

Sample preparation of brain homogenate with liquid-liquid extraction

Brain samples were thawed, weighed, and homogenized with the same volume of ice-cold water with a hand homogenizer. Rat brain homogenate (100 μL) with or without P-bi-TAT (150 ng/μL) and IS (P-mono-TAT, 150 ng/μL) was vortex-mixed with 1 mL of acetonitrile/water (80/20 v/v) for 30 min. After centrifugation, solvent was evaporated under a nitrogen stream at 50 °C. Extracts were reconstituted with 50 μL of acetonitrile/water (80/20 v/v).

LC-MS/MS instrumentation

An API-4000 mass spectrometer (Sciex, Framingham, MA) equipped with Shimadzu UPLC system (Kyoto, Japan) was used for LC-MS/MS analyses. A Kinetex 2.6 μm Biphenyl 100 LS column (50 × 2.1 mm, Phenomenex, Torrance, CA) was used for reversed-phase separation. Mobile phases were (A) water containing 0.1% formic acid and 5% acetonitrile and (B) acetonitrile with 0.1% formic acid. The flow rate was 0.5 mL/min and the gradient was linear from 0% B for 1 min to 95% B for 5-7 min. The column oven temperature was 40 °C and the injection volume was 5 μL. Electro-spray ionization (ESI) was used in positive MRM mode. Mass transitions for analytes were as follows: Q1/Q3, 1358.1/582.4 (P-bi-TAT); 1402.1/626.4 (IS). The operative parameters of the mass spectrometer were as follows: declustering potentials (DP), 130 V; entrance potentials (EP), 10; collision energies (CE), 51 eV; collision cell exit potential (CXP), 18 V; curtain gas (CUR), 30 psi; gas 1 (GS1, nebulizer gas) 30 psi; gas 2 (GS2, heater gas) 50 psi; ion spray voltage (IS), 5000 V; temperature (TEM), 400 °C; collision activate dissociation (CAD) gas, 10 psi; dwell time, 150 ms. Nitrogen was used for the gases.

A standard curve was obtained using standard solutions of P-bi-TAT with concentrations of 10.24, 25.6, 64, 160, 400, and 1000 ng/mL.

Determination of recovery efficient and matrix effect

Recovery efficient was calculated with the response of extracts from spiked plasma sample (sample) and blank plasma sample (blank) with spike after extract as follows:

$$ \mathrm{Recovery}\ \mathrm{efficient}\ \left(\%\right)=\mathrm{Response}\ \mathrm{in}\ \mathrm{sample}/\mathrm{Response}\ \mathrm{in}\ \mathrm{blank}\times 100 $$

Similarly, matrix effect was calculated with the response of extracts from spiked plasma sample (sample) and standard solution (standard) as follows:

$$ \mathrm{Matrix}\ \mathrm{effect}\ \left(\%\right)=\mathrm{Response}\ \mathrm{in}\ \mathrm{sample}/\mathrm{Response}\ \mathrm{in}\ \mathrm{standard}\times 100 $$

Determination of limit of quantification (LOQ) and limit of quantification (LOD)

Standard curve was obtained using standard solutions of β-C-TAT with IS. The limit of quantification (LOQ) and the limit of quantification (LOD) was determined using data of six injections of a standard solution (1000 ng/mL). LOQ and LOD were calculated using the standard deviations (SD) of signals for the analyte and the slope of linear regression curve as follows:

$$ \mathrm{LOQ}=10\times \mathrm{SD}/\mathrm{slope} $$
$$ \mathrm{LOD}=3\times \mathrm{SD}/\mathrm{slope} $$

Results

Development of bioanalytical method for P-bi-TAT

The ionization of P-bi-TAT was studied in positive and negative modes. Deprotonated pseudo-molecular ions [M-2H]2− of P-bi-TAT were detected in negative mode (Fig. 2). The mean difference in m/z of ions is 22.0, which is corresponding to the half of an ethylene oxide unit (44 DA). The calculated MW of P-bi-TAT using m/z of deprotonated pseudo-molecular ions was consistent with the theoretical values and ranged from 4899.6 (n = 74) to 5998.2 (n = 99). The mathematical mean MW of P-bi-TAT calculated with strong thirteen m/z of deprotonated pseudo-molecular ions was 5736.0 and is close to the theoretical Mw of P-bi-TAT, 5620. The mass spectrum of P-bi-TAT in negative mode is useful for identification of the molecule; however, the intensities of deprotonated molecular ions [M-2H]2− were very weak, so they were not applicable to MRM.

Fig. 2
figure2

Mass spectrum of P-bi-TAT in negative ESI scan mode

When positive mode was applied, multiply protonated pseudo-molecular ions and fragmental protonated molecular ions [MH]+ of P-bi-TAT were detected by in-source CID (Fig. 3). The calculated MW of P-bi-TAT using m/z of multiply protonated pseudo-molecular ions (z = 5) was consistent with the theoretical values and ranged from 5077.0 (n = 78) to 6130.0 (n = 102). The mathematical mean MW of P-bi-TAT calculated with strong thirteen m/z of deprotonated pseudo-molecular ions was 5605.3 and is close to the theoretical Mw of P-bi-TAT, 5620. The region 1 of mass spectrum of P-bi-TAT in positive mode is useful for identification of the molecule; however, the intensities of multiply protonated molecular ions [M+nH]n+ were weak, so they were not applicable to MRM.

Fig. 3
figure3

Mass spectrum of P-bi-TAT in positive ESI scan mode

Next, DP was increased from 45 V to 130 V to enhance in-source CID, fragmental protonated molecular ions [MH]+ of P-bi-TAT were dominantly detected (Fig. 4). The mean difference in m/z of ions is 44.0, which is equal to the ethylene oxide unit (44 da). The fragmental protonated ions ranged from 1182.1 (n = 8) to 2415.9 (n = 36). Two of the strong fragmental protonated ions (m/z 1358, 1402) were selected as precursor ions of MRM. MRM optimization was performed for each precursor ion, and MS parameters were optimized with flow injection analysis (FIA) optimization and an LC-MS/MS method was developed with the biphenyl column. The MRM was also applied to the IS because of the similarity in chemical structures of P-bi-TAT and IS. One of each MRM was used for quantification for P-bi-TAT and IS, and the other was used as a qualifier for each. Under these conditions, the retention times for P-bi-TAT and IS were 5.7 and 5.2 min, respectively (Fig. 5).

Fig. 4
figure4

Full scan mass spectrum (Q1MS) of P-bi-TAT by in-source CID in positive mode

Fig. 5
figure5

Typical LC-MS/MS for P-bi-TAT (5.7 min) and IS (5.2 min) in positive mode

The LC-MS/MS method for P-bi-TAT with IS was linear from a concentration of 25 to 1000 ng/mL (Fig. 6). The method detection limit (MDL) for P-bi-TAT was estimated to be 30 ng/mL under these conditions. Recovery efficiency was 76% and the matrix effect was 19%.

Fig. 6
figure6

Standard curve for P-bi-TAT with IS by positive LC-MS/MS

PK and biodistribution of P-bi-TAT in rats and monkeys

The bioanalytical method was applied to PK studies in rats and monkeys. The time course of P-bi-TAT levels in rat plasma (over 48 h) is shown in Fig. 7 and in monkey plasma (on day 1 and day 7) in Fig. 8. Cmax and area under the curve (AUC) in rats and monkeys (day 1), PK parameters in rats and monkeys, and the dose proportionality ratios of Cmax and AUC are shown in Table 1.

Fig. 7
figure7

Time course of plasma levels of P-bi-TAT (10 mg/kg) in rats n = 4. Error bars represent standard errors of the mean

Fig. 8
figure8

Time courses of plasma levels of P-bi-TAT in monkeys on day 1 and day 7, n = 2 for female and n = 2 for males. Low dose 25 mg/kg body weight, high dose 82.5 mg/kg body weight. Error bars represent standard errors of the mean. Day 1, low dose: closed circles; day 7, low dose: open circles; day 1, high dose: closed squares; day 7, high dose: open squares

Table 1 Parameters in rats (n = 4) and monkeys (n = 2 female and 2 male) dosed with P-bi-TAT

Biodistribution of P-bi-TAT in the brain of rats varied from 7 (low) to 90 (high) ng/g brain weight (Fig. 9). The levels of P-bi-TAT in the brain were dose-dependent.

Fig. 9
figure9

P-bi-TAT levels in the brain of rats (n = 4) at low (1 mg/kg), medium (3 mg/kg), and high (10 mg/kg) doses. Error bars represent standard errors of the mean

Discussion

Development of bioanalytical method for P-bi-TAT

P-bi-TAT has both hydrophilic (PEG) and lipophilic (triazole tetrac) structures with high affinity for αvβ3 integrin (Rajabi et al. 2019), resulting in favorable pharmaceutical properties as a targeted anticancer therapeutic. However, it was difficult to develop a quantitative method in MRM in both positive and negative modes because there are multiple molecular weights of P-bi-TAT and multiple charged ions (n = 1-5) simultaneously formed by ESI. In the positive mode, it shows multiple-charged ions and fragmented ions. Multiple-charged ions are dominant under the low declustering potential (DP, ~45 V); however, those ions were too weak as a precursor ion to develop an MRM method. The intensities of fragmented ions showed a maximum under high DP (~130 V), so we were able to develop the MRM method under those conditions. The LOQ and recovery of the bioanalytical method was satisfactory.

In the negative mode, deprotonated pseudo-molecular ions [M-2H]2− of P-bi-TAT were not strong enough for MRM method development. However, they were useful for calculating the exact mass of individual P-bi-TAT by multiplying m/z of the deprotonated ion by z plus z (Mw = m/z × z = z).

PK and biodistribution of P-bi-TAT in rats and monkeys

The bioanalytical method was successfully applied to the PK studies in rats and monkeys. Tmax values were the same value of 6 h in rats and monkeys. The Cmax and AUC values in rats were similar to those in monkeys at low dose. These results of Cmax and AUC suggest that monkeys may be a better model animal than rats for P-bi-TAT, and higher equivalent doses of P-bi-TAT may be required for monkeys (and possibly humans) than for rats to achieve the targeted concentrations in plasma and tissues. Half-lives (T1/2) were longer in monkeys than in rats. Human T1/2 was estimated to be 49 h using a simple allometric formula, Y = a(Xb), where Y is human T1/2; X is rat T1/2; and a and b are constants (Sarver et al. 1997). The long half-life in monkeys and possibly in humans suggests the prolonged efficacy of P-bi-TAT as a targeted anticancer treatment (Miyazaki et al. 2021).

The dose ratio between low and high was 3.3 in the monkey study. The results in dose proportionality ratios for Cmax and AUC in the monkey study suggest that the bioavailability of P-bi-TAT is dose-dependent within the dose range administered. Accumulation of P-bi-TAT in target organs and in off-target organs, such as liver and kidney, needs to be investigated further.

The dose-dependent biodistribution of P-bi-TAT in the brain suggests that it is applicable to the therapeutics of brain cancers. Fernandes and coworkers reported that PEGylated caffeic acid and ferulic acid (MW ~1000) were permeable across hCMEC/D3 monolayer cells, a model of the blood-brain barrier, by transcellular passive diffusion due to the hydrophobicity (Fernandes et al. 2018). Yang et al. showed that a PEGylated amifostine had cytoprotective effects in vitro (Yang et al. 2016). Fleming et al. reported that the chemotherapy drug Camptothecin conjugated with PEG3400 was delivered into the brain of rats (Fleming et al. 2004). The biodistribution of P-bi-TAT in the brains in the current study may be attributed to the involvement of transthyretin (TTR) and thyroid hormone transporters, such as MCTs and OATPs (Bernal et al. 2015; Horn et al. 2013; Kinne et al. 2010), in addition to passive diffusion because of the high molecular weight of P-bi-TAT (MW > 5000).

Conclusion

The PK parameters and biodistribution of P-bi-TAT in rats and monkeys showed its preferable characteristics as a targeted anticancer pharmaceutical. A quantitative bioanalytical method for P-bi-TAT was developed using LC-MS/MS and SPE. P-bi-TAT showed the strongest MRM intensity in the positive mode under ion-source CID conditions. The LOQ for P-bi-TAT was 30 ng/μL and the recovery efficiency was 76%. The bioanalytical method was applied to PK studies of P-bi-TAT and results suggest that P-bi-TAT is a promising candidate as a targeted anticancer drug. Its efficacies on multiple cancer types, accumulation in organs, toxicokinetic, and pharmacodynamics need to be further investigated.

Availability of data and materials

All data are available and stored at Pharmaceutical Research Institute (PRI)intranet. All materials related to the project are available at PRI laboratories.

References

  1. Anthony C, Mladkova-Suchy N, Adamson DC (2019) The evolving role of antiangiogenic therapies in glioblastoma multiforme: current clinical significance and future potential. Expert Opin Investig Drugs 28(9):787–797. https://doi.org/10.1080/13543784.2019.1650019

    CAS  Article  PubMed  Google Scholar 

  2. Bernal J, Guadaño-Ferraz A, Morte B (2015) Thyroid hormone transporters--functions and clinical implications. Nat Rev Endocrinol 11(7):406–417. https://doi.org/10.1038/nrendo.2015.66

    CAS  Article  PubMed  Google Scholar 

  3. Fernandes C, Pinto M, Martins C, Gomes MJ, Sarmento B, Oliveira PJ, Remião F, Borges F (2018) Development of a PEGylated-based platform for efficient delivery of dietary antioxidants across the blood-brain barrier. Bioconjug Chem 29(5):1677–1689. https://doi.org/10.1021/acs.bioconjchem.8b00151

    CAS  Article  PubMed  Google Scholar 

  4. Fleming AB, Haverstick K, Saltzman WM (2004) In vitro cytotoxicity and in vivo distribution after direct delivery of PEG-camptothecin conjugates to the rat brain. Bioconjug Chem 15(6):1364–1375. https://doi.org/10.1021/bc034180o

    CAS  Article  PubMed  Google Scholar 

  5. Fox BM, Janssen A, Estevez-Ordonez D, Gessler F, Vicario N, Chagoya G, Elsayed G, Sotoudeh H, Stetler W, Friedman GK, Bernstock JD (2019) SUMOylation in glioblastoma: a novel therapeutic target. Int J Mol Sci 20(8):1853. https://doi.org/10.3390/ijms20081853

    CAS  Article  PubMed Central  Google Scholar 

  6. Henley SJ, Ward EM, Scott S, Ma J, Anderson RN, Firth AU, Thomas CC, Islami F, Weir HK, Lewis DR, Sherman RL, Wu M, Benard VB, Richardson LC, Jemal A, Cronin K, Kohler BA (2020) Annual report to the nation on the status of cancer, part I: National cancer statistics. Cancer 126(10):2225–2249. https://doi.org/10.1002/cncr.32802

    Article  PubMed  Google Scholar 

  7. Horn S, Kersseboom S, Mayerl S, Müller J, Groba C, Trajkovic-Arsic M, Ackermann T, Visser TJ, Heuer H (2013) Tetrac can replace thyroid hormone during brain development in mouse mutants deficient in the thyroid hormone transporter mct8. Endocrinology 154(2):968–979. https://doi.org/10.1210/en.2012-1628

    CAS  Article  PubMed  Google Scholar 

  8. Kinne A, Kleinau G, Hoefig CS, Grüters A, Köhrle J, Krause G, Schweizer U (2010) Essential molecular determinants for thyroid hormone transport and first structural implications for monocarboxylate transporter 8. J Biol Chem 285(36):28054–28063. https://doi.org/10.1074/jbc.M110.129577

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  9. Laquintana V, Trapani A, Denora N, Wang F, Gallo JM, Trapani G (2009) New strategies to deliver anticancer drugs to brain tumors. Expert Opin Drug Deliv 6(10):1017–1032. https://doi.org/10.1517/17425240903167942

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  10. Le Rhun E, Preusser M, Roth P, Reardon DA, van den Bent M, Wen P, Reifenberger G, Weller M (2019) Molecular targeted therapy of glioblastoma. Cancer Treat Rev 80:101896. https://doi.org/10.1016/j.ctrv.2019.101896

    CAS  Article  PubMed  Google Scholar 

  11. Lin HY, Chin YT, Nana AW, Shih YJ, Lai HY, Tang HY, Leinung M, Mousa SA, Davis PJ (2016) Actions of l-thyroxine and nano-diamino-tetrac (Nanotetrac) on PD-L1 in cancer cells. Steroids. 114:59–67. https://doi.org/10.1016/j.steroids.2016.05.006

    Article  PubMed  Google Scholar 

  12. Lin SJ, Chin YT, Ho Y, Chou SY, Sh Yang YC, Nana AW, Su KW, Lim YT, Wang K, Lee SY, Shih YJ, Chen YR, Whang-Peng J, Davis PJ, Lin HY, Fu E (2018) Nano-diamino-tetrac (NDAT) inhibits PD-L1 expression which is essential for proliferation in oral cancer cells. Food Chem Toxicol 120:1–11

    CAS  Article  Google Scholar 

  13. Maeda H, Tsukigawa K, Fang J (2016) A retrospective 30 years after discovery of the enhanced permeability and retention effect of solid tumors: next-generation chemotherapeutics and photodynamic therapy--problems, solutions, and prospects. Microcirculation 23(3):173–182. https://doi.org/10.1111/micc.12228

    CAS  Article  PubMed  Google Scholar 

  14. Miyazaki T, Maiti M, Hennessy M, Chang T, Kuo P, Addepalli M, Obalapur P, Sheibani S, Wilczek J, Pena R, Quach P, Cetz J, Moffett A, Tang Y, Kirk P, Huang J, Sheng D, Zhang P, Rubas W, Madakamutil L, Kivimäe S, Zalevsky J (2021) NKTR-255, a novel polymer-conjugated rhIL-15 with potent antitumor efficacy. J Immunother Cancer 9(5):e002024. https://doi.org/10.1136/jitc-2020-002024

    Article  PubMed  PubMed Central  Google Scholar 

  15. Rajabi M, Godugu K, Sudha T, Bharali DJ, Mousa SA (2019) Triazole modified tetraiodothyroacetic acid conjugated to polyethylene glycol: high affinity thyrointegrin αvβ3 antagonist with potent anticancer activities in glioblastoma multiforme. Bioconjug Chem 30(12):3087–3097. https://doi.org/10.1021/acs.bioconjchem.9b00742

    CAS  Article  PubMed  Google Scholar 

  16. Sarver JG, White D, Erhardt P, Bachmann K (1997) Estimating xenobiotic half-lives in humans from rat data: influence of log P. Environ Health Perspect 105(11):1204–1209. https://doi.org/10.1289/ehp.971051204

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  17. Sudha T, Bharali DJ, Sell S, Darwish NHE, Davis PJ, Mousa SA (2017a) Nanoparticulate tetrac inhibits growth and vascularity of glioblastoma xenografts. Horm Cancer 8(3):157–165. https://doi.org/10.1007/s12672-017-0293-6

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  18. Sudha T, Bharali DJ, Yalcin M, Darwish NH, Coskun MD, Keating KA, Lin HY, Davis PJ, Mousa SA (2017b) Targeted delivery of cisplatin to tumor xenografts via the nanoparticle component of nano-diamino-tetrac. Nanomedicine (London) 12(3):195–205. https://doi.org/10.2217/nnm-2016-0315

    CAS  Article  Google Scholar 

  19. Warrack BM, Redding BP, Chen G, Bolgar MS (2013) Determination of the molecular weight of poly(ethylene glycol) in biological samples by reversed-phase LC-MS with in-source fragmentation. Anal Bioanal Chem 405(12):4283–4287. https://doi.org/10.1007/s00216-013-6795-3

    CAS  Article  PubMed  Google Scholar 

  20. Xu J, Murphy SL, Kockanek KD, Arias E (2020) Mortality in the United States, 2018. NCHS Data Brief 355:1–8

    Google Scholar 

  21. Yang X, Ding Y, Ji T, Zhao X, Wang H, Zhao X, Zhao R, Wei J, Qi S, Nie G (2016) Improvement of the in vitro safety profile and cytoprotective efficacy of amifostine against chemotherapy by PEGylation strategy. Biochem Pharmacol 108:11–21. https://doi.org/10.1016/j.bcp.2016.02.014

    CAS  Article  PubMed  Google Scholar 

  22. Yin L, Ren T, Zhao S, Shi M, Gu J (2020) Comparative pharmacokinetic study of PEGylated gemcitabine and gemcitabine in rats by LC-MS/MS coupled with pre-column derivatization and MSALL technique. Talanta 206:120184. https://doi.org/10.1016/j.talanta.2019.120184

    CAS  Article  PubMed  Google Scholar 

Download references

Acknowledgements

Special thanks to both NanoPharmaceuticals LLC and from the Pharmaceutical Research Institute for their funding. Special thanks to PRI chemists and biologists for their teamwork.

Funding

Funding was received from both NanoPharmaceuticals LLC and from the Pharmaceutical Research Institute at Albany College of Pharmacy and Health Sciences.

Author information

Affiliations

Authors

Contributions

Kazutoshi Fujioka contributed to the analytical and bioanalytical method development, validation, and drafted the manuscript; Kavitha Godugu contributed to the animal studies for PK and biodistribution; and Shaker A. Mousa contributed the study design, concepts, data review, supervision, and edits of the manuscript. The authors read and approved the final manuscript.

Corresponding author

Correspondence to Shaker A. Mousa.

Ethics declarations

Competing interests

S.A. Mousa has a patent related to P-bi-TAT and he is a founder of NanoPharmaceuticals LLC, which is developing anticancer drugs. All other authors declare no conflicts of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Fujioka, K., Godugu, K. & Mousa, S.A. Pharmacokinetics and biodistribution of a novel anticancer thyrointegrin αvβ3 antagonist: triazole modified tetraiodothyroacetic acid conjugated to polyethylene glycol (P-bi-TAT). AAPS Open 7, 2 (2021). https://doi.org/10.1186/s41120-021-00036-z

Download citation

Keywords

  • PEGylated
  • Triazole tetrac
  • Integrin αvβ3 antagonist
  • Pharmacokinetic
  • Toxicokinetic
  • LC-MS/MS
  • Anti-angiogenic
  • Blood-brain barrier