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Table 4 Three-month assessment of microbial death at 15 s for alcohol solutions containing different additives

From: Designing an ideal alcohol-based hand sanitizer: in vitro antibacterial responses of ethanol and isopropyl alcohol solutions to changing composition

Additives

Alcohol

Microbial death (%)

S. aureus

E. coli

Fresh

3 months

Fresh

3 months

Benzalkonium chloride (0.05%)

Ethanol

95.22 ± 0.74

90.81 ± 1.32*

100.00 ± 0.00

96.49 ± 0.71*

Isopropyl alcohol

100.00 ± 0.00†

97.67 ± 0.56

100.00 ± 0.00

98.98 ± 0.68†

Carrot extract

Ethanol

96.94 ± 0.92

96.94 ± 1.53

98.98 ± 0.34

98.30 ± 0.34

Isopropyl alcohol

63.48 ± 0.76†

51.96 ± 1.12*

95.13 ± 0.86†

90.59 ± 0.86*†

Cucumber extract

Ethanol

76.22 ± 0.76

70.71 ± 0.56*

100.00 ± 0.00

98.98 ± 0.68

Isopropyl alcohol

56.13 ± 0.57†

23.41 ± 2.81*

98.19 ± 0.52†

93.77 ± 0.52*†

Aloe vera extract

Ethanol

91.30 ± 1.12

84.07 ± 0.77*

88.21 ± 0.86

86.17 ± 1.75*

Isopropyl alcohol

68.87 ± 0.93†

58.33 ± 1.53*

98.41 ± 0.52†

97.28 ± 0.68†

  1. Values represent means ± SD. Values with * indicate a significant difference (P < 0.05) from fresh sample for the same alcohol/additive/organism. Values with † indicate significant difference (P < 0.05) from ethanol for the same additive/organism/test time. N = 3