Table 1 Levels of variables in 23 full factorial design
Independent variables | ||
|---|---|---|
Factors | Levels, coded (actual) | |
Low | High | |
X1: Drug to span 60 molar ratio | − 1 (1:4) | + 1 (1:8) |
X2: Volume of hydration (PBS 7.4) | − 1 (10 ml) | + 1 (100 ml) |
X3: Stirring speed | − 1 (1000 rpm) | + 1 (2500 rpm) |
Dependent variables | ||
Y1:Average vesicle size (nm) Y2: Entrapment efficiency (%) | ||