Table 4 Design matrix and response for 23 factorial design batches [X1—drug to span 60 molar ratio, X2—volume of hydration (PBS 7.4), X3—stirring speed, Y1—average vesicle size (nm), and Y2—entrapment efficient (%)]
Batch no. | Independent variables in coded form | ||||
|---|---|---|---|---|---|
X1 | X2 | X3 | Y1 | Y2 | |
B1 | − 1 | − 1 | − 1 | 240.0 ± 1.1 | 60.5 ± 1.1 |
B2 | + 1 | − 1 | − 1 | 208.6 ± 0.9 | 59.1 ± 3.1 |
B3 | − 1 | + 1 | − 1 | 315.0 ± 2.1 | 61.4 ± 0.9 |
B4 | + 1 | + 1 | − 1 | 340.7 ± 2.9 | 64.9 ± 0.3 |
B5 | − 1 | − 1 | + 1 | 65.1 ± 0.6 | 55.3 ± 0.5 |
B6 | + 1 | − 1 | + 1 | 45.0 ± 0.6 | 48.3 ± 0.8 |
B7 | − 1 | + 1 | + 1 | 150.6 ± 1.5 | 84.6 ± 1.9 |
B8 | + 1 | + 1 | + 1 | 179.4 ± 1.9 | 88.5 ± 1.6 |
B9 | 0 | 0 | 0 | 105.8 ± 1.2 | 70.8 ± 2.5 |
B10 | 0 | 0 | 0 | 115.5 ± 1.1 | 72.1 ± 1.7 |
B11 | 0 | 0 | 0 | 110.0 ± 0.8 | 70.9 ± 1.4 |
B12 (checkpoint batch) | − 0.5 | − 0.5 | − 0.5 | 190.7 ± 1.1 | 65.6 ± 1.8 |
B13 (optimized batch) | − 0.5 | + 1 | + 1 | 196.6 ± 0.9 | 78.31 ± 1.4 |