A bridging assay for detection and characterization of anti-drug antibodies to dostarlimab, a new anti-PD-1 therapeutic monoclonal antibody

Patterson, Marilyn; Beausang, Lee Anne; Rup, Bonita; Bowsher, Ronald R.; Krug, Kim; Melhem, Murad; Lu, Sharon

doi:10.1186/s41120-021-00045-y

AAPS Open

Table 2 Independent single-plate formats for assay runs

From: A bridging assay for detection and characterization of anti-drug antibodies to dostarlimab, a new anti-PD-1 therapeutic monoclonal antibody

Group A	1	2	3	4	5	6	7	8	9	10	11	12
A	NC		NC		Cut point sample 4		Cut point sample 8		Cut point sample 12		Cut point sample 16
B	NC		NC		Cut point sample 4		Cut point sample 8		Cut point sample 12		Cut point sample 16
C	LPC^a		Cut point sample 1		Cut point sample 5		Cut point sample 9		Cut point sample 13		Cut point sample 17
D	LPC^a		Cut point sample 1		Cut point sample 5		Cut point sample 9		Cut point sample 13		Cut point sample 17
E	MPC		Cut point sample 2		Cut point sample 6		Cut point sample 10		Cut point sample 14		NC
F	MPC		Cut point sample 2		Cut point sample 6		Cut point sample 10		Cut point sample 14		NC
G	HPC		Cut point sample 3		Cut point sample 7		Cut point sample 11		Cut point sample 15		NC
H	HPC		Cut point sample 3		Cut point sample 7		Cut point sample 11		Cut point sample 15		NC
Group B	1	2	3	4	5	6	7	8	9	10	11	12
A	NC		NC		Cut point sample 21		Cut point sample 25		Cut point sample 29		Cut point sample 33
B	NC		NC		Cut point sample 21		Cut point sample 25		Cut point sample 29		Cut point sample 33
C	LPC^a		Cut point sample 18		Cut point sample 22		Cut point sample 26		Cut point sample 30		Cut point sample 34
D	LPC^a		Cut point sample 18		Cut point sample 22		Cut point sample 26		Cut point sample 30		Cut point sample 34
E	MPC		Cut point sample 19		Cut point sample 23		Cut point sample 27		Cut point sample 31		NC
F	MPC		Cut point sample 19		Cut point sample 23		Cut point sample 27		Cut point sample 31		NC
G	HPC		Cut point sample 20		Cut point sample 24		Cut point sample 28		Cut point sample 32		NC
H	HPC		Cut point sample 20		Cut point sample 24		Cut point sample 28		Cut point sample 32		NC
Group C	1	2	3	4	5	6	7	8	9	10	11	12
A	NC		NC		Cut point sample 38		Cut point sample 42		Cut point sample 46		Cut point sample 50
B	NC		NC		Cut point sample 38		Cut point sample 42		Cut point sample 46		Cut point sample 50
C	LPC^a		Cut point sample 35		Cut point sample 39		Cut point sample 43		Cut point sample 47		Cut point sample 51
D	LPC^a		Cut point sample 35		Cut point sample 39		Cut point sample 43		Cut point sample 47		Cut point sample 51
E	MPC		Cut point sample 36		Cut point sample 40		Cut point sample 44		Cut point sample 48		NC
F	MPC		Cut point sample 36		Cut point sample 40		Cut point sample 44		Cut point sample 48		NC
G	HPC		Cut point sample 37		Cut point sample 41		Cut point sample 45		Cut point sample 49		NC
H	HPC		Cut point sample 37		Cut point sample 41		Cut point sample 45		Cut point sample 49		NC

^aLPC concentration is 40 ng/mL; white cell, unspiked sample; gray cell, spiked with 405 ng/mL unlabeled dostarlimab

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