Screening assay | |||||||||||||||||
Batch 30 plate 1 | Batch 30 plate 2 | ||||||||||||||||
Block | 25 min | 30 min | 35 min | 30 min | 30 min | 30 min | 30 min | 30 min | |||||||||
MM | 180 min | 180 min | 180 min | 170 min | 190 min | 180 min | 180 min | 180 min | |||||||||
Sample | 60 min | 60 min | 60 min | 60 min | 60 min | 50 min | 60 min | 70 min | |||||||||
Mean ECL | % Diff | Baseline mean ECL | Mean ECL | % Diff | Mean ECL | % Diff | Mean ECL | % Diff | Mean ECL | % Diff | Baseline mean ECL | Mean ECL | % Diff | ||||
HPC | 146021 | 2.3 | 142677 | 146095 | 2.4 | 142399 | −0.2 | 148013 | 3.7 | 180810 | 1.4 | 178324 | 180245 | −1.1 | |||
MPC | 2869 | 1.1 | 2839 | 2847 | 0.3 | 2805 | −1.2 | 2848 | 0.3 | 3630 | −2.4 | 3718 | 3670 | 1.3 | |||
LPCa | 341 | 1.9 | 334 | 339 | 1.5 | 336 | 0.4 | 337 | 0.9 | 400 | 1.1 | 395 | 396 | −0.3 | |||
NC | 106 | 6.6 | 99 | 103 | 3.5 | 105 | 6.1 | 102 | 3.0 | 104 | 4.0 | 100 | 103 | −2.9 | |||
NC | 109 | −0.5 | 109 | 111 | 1.4 | 115 | 5.5 | 109 | 0.0 | 110 | −3.9 | 115 | 111 | 3.2 | |||
NC | 101 | −1.5 | 103 | 100 | −2.4 | 103 | 0.0 | 101 | −2.0 | 101 | −1.0 | 102 | 103 | −1.5 | |||
NC | 111 | 1.4 | 109 | 107 | −2.3 | 110 | 0.9 | 108 | −0.9 | 111 | −0.9 | 112 | 111 | 0.9 | |||
NC | 105 | 1.0 | 104 | 102 | −2.4 | 106 | 1.4 | 100 | −3.8 | 103 | −1.9 | 105 | 107 | −1.9 | |||
Confirmatory assay | |||||||||||||||||
Batch 31 plate 1 | Batch 31 plate 2 | ||||||||||||||||
Block | 30 min | 30 min | 30 min | 30 min | 30 min | SGP | 65 min | 60 min | 55 min | 60 min | |||||||
MM | 180 min | 177 min | 187 min | 180 min | 180 min | MM | 180 min | 180 min | 180 min | 180 min | |||||||
Drug | 60 min | 60 min | 60 min | 57 min | 65 min | Drug | 60 min | 60 min | 60 min | 60 min | |||||||
Mean ECL | |||||||||||||||||
No drug | With drugs | Mean ECL | % Diff | Mean ECL | % Diff | Mean ECL | % Diff | Mean ECL | % Diff | Mean ECL | % Diff | BL mean ECL | Mean ECL | % Diff | Mean ECL no drug | ||
HCP | 136898 | 135 | 134 | −0.7 | 134 | −1.1 | 134 | −0.7 | 135 | −0.4 | 125 | 1.6 | 123 | 122 | −1.2 | 144616 | |
MPC | 2898 | 90 | 88 | −2.8 | 89 | −1.1 | 90 | 0.0 | 91 | 1.1 | 85 | 3.00 | 83 | 82 | −0.6 | 2947 | |
LPCa | 363 | 98 | 94 | −4.1 | 94 | −3.6 | 97 | −0.5 | 96 | −1.5 | 92 | 4.5 | 88 | 90 | 2.3 | 340 | |
NC | 110 | 91 | 86 | −5.5 | 87 | −4.9 | 91 | −0.5 | 90 | −1.1 | 83 | 0.6 | 83 | 81 | −1.8 | 103 | |
NC | 116 | 97 | 95 | −1.6 | 95 | −1.6 | 96 | −0.5 | 99 | 2.6 | 92 | 1.7 | 91 | 91 | 0.6 | 114 | |
NC | 109 | 91 | 90 | −1.6 | 90 | −1.1 | 92 | 1.1 | 95 | 3.8 | 85 | 3.7 | 82 | 85 | 3.7 | 104 | |
NC | 117 | 98 | 97 | −1.5 | 98 | 0.0 | 100 | 1.5 | 103 | 5.1 | 93 | 1.6 | 91 | 94 | 2.7 | 111 | |
NC | 110 | 97 | 89 | −7.8 | 91 | −5.7 | 95 | −2.1 | 95 | −1.6 | 87 | 3.0 | 85 | 86 | 1.2 | 106 |