Table 8 Comparison of the method sensitivity without or at different acid dissociation concentration
No AA 100 μg/mL free drug | 300 mM AA 100 μg/mL free drug | 600 mM AA 100 μg/mL free drug | 900 mM AA 100 μg/mL free drug | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
PC conc. (ng/mL) | Signal | S/N | % Recovery mean | Signal | S/N | % Recovery mean | Signal | S/N | % Recovery mean | Signal | S/N | % Recovery mean |
5000 | 1060 | 10.9 | 102.6 | 1435 | 13.2 | 104.8 | 1634 | 6.4 | 103.6 | 2241 | 1.4 | 100.0 |
2500 | 574 | 5.9 | 98.5 | 713 | 6.5 | 92.6 | 912 | 3.5 | 95.2 | 1208 | 0.8 | NaN |
1250 | 347 | 3.6 | 99.7 | 402 | 3.7 | 106.8 | 621 | 2.4 | 101.8 | 1646 | 1.0 | 380.0 |
625 | 217 | 2.2 | 91.1 | 211 | 1.9 | 105.7 | 434 | 1.7 | 91.9 | 1403 | 0.9 | NaN |
312.5 | 174 | 1.8 | 112.2 | 141 | 1.3 | 104.9 | 374 | 1.5 | 113.4 | 1610 | 1.0 | 1508.9 |
156.25 | 131 | 1.4 | 87.8 | 95 | 0.9 | NaN | 307 | 1.2 | 72.7 | 1392 | 0.9 | NaN |
78.125 | 131 | 1.4 | 175.6 | 143 | 1.3 | 434.3 | 341 | 1.3 | 300.8 | 1682 | 1.1 | 6116.9 |
0 | 97 | 1.0 | NaN | 109 | 1.0 | NaN | 257 | 1.0 | NaN | 1589 | 1.0 | NaN |