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Fig. 7 | AAPS Open

Fig. 7

From: Impact of charge patches on tumor disposition and biodistribution of therapeutic antibodies

Fig. 7

Experimental assessment of interstitial fluid (ISF) concentrations confirms relative enrichment and binding availability of positive charge-patch variant (see Supplemental Figure 3 for a schematic illustration the centrifugation to obtain interstitial fluid). a Antibody levels in muscle interstitial fluid in C57BL/6 mice as determined by LC-MS. b ISF-to-serum ratios in muscle, calculated from the experimental data shown in a. c For comparison to b, ISF-to-serum ratios in muscle were derived by the method described in (Chang et al. 2020) for the study in tumor-bearing BALB/c mice. d The ISF-to-serum ratio in tumor, also derived using the derivation approach of (Chang et al. 2020). e Interstitial fluid levels in skin following the centrifugation protocol in C57BL/6 mice. f ISF-to-serum ratios calculated from the skin interstitial fluid levels as shown in e

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