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Table 1 Gradient program of analytical chromatography

From: The degradation of poloxamer 188 in buffered formulation conditions

Time [min] Eluent A [%] Eluent B [%]
0.0 98 2
1.0 98 2
100.0 50 50
101.0 42 58
117.0 36 64
119.5 10 90
122.5 10 90
124.5 0 100
131.0 0 100
133.0 98 2
145.0 98 2