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Table 1 Gradient program of analytical chromatography

From: The degradation of poloxamer 188 in buffered formulation conditions

Time [min]

Eluent A [%]

Eluent B [%]

0.0

98

2

1.0

98

2

100.0

50

50

101.0

42

58

117.0

36

64

119.5

10

90

122.5

10

90

124.5

0

100

131.0

0

100

133.0

98

2

145.0

98

2