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Table 3 Gradient program of P188 fractionation

From: The degradation of poloxamer 188 in buffered formulation conditions

Time [min] Eluent A [%] Eluent B [%]
0.0 50 50
10.0 50 50
235.0 36 64
245.0 0 100
255.0 0 100
265.0 50 50
280.0 50 50