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Fig. 1 | AAPS Open

Fig. 1

From: Heat pre-treatment can abolish anti-drug antibody interference in ligand binding pharmacokinetic assays

Fig. 1

Effect of heat pre-treatments on recovery of pAB-treated spiked samples. pAB towards the analyte (NNC9204-1177) was added to the spiked samples to mimic ADA interference. Samples were analyzed by a traditional ECL assay or an ECL assay with one of five different heat pre-treatments. Recovery was calculated as the ratio between the measured concentration and the nominal concentration in percent and was plotted against the pAB concentration. The results from three independent experiments are included. Dotted lines indicate acceptance criterion boundaries. Error bars indicate standard deviation. For each assay, the correlation between recovery and pAB concentration was calculated by the Spearman correlation test. *P < 0.05, ****P < 0.0001

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