Fig. 2

Difference in the measured concentration caused by heat pre-treatment. a Forty-one ADA-positive and 23 ADA-negative PK study samples from a total of 60 cynomolgus monkeys were each analyzed by a traditional ECL assay and an ECL assay with heat pre-treatment (analyte NNC9204-1177). b The same approach was used on PK study samples with a different analyte (NNC0247-0829), and 7 ADA-positive and 22 ADA-negative samples from a total of 29 minipigs were analyzed. In both graphs, the difference in measured concentration between the two assays is plotted as fold change for each sample. Fold change is calculated as the ratio between the measured concentration with heat pre-treatment and the measured concentration with the traditional assay. Dotted lines indicate acceptance criterion boundaries. Error bars indicate standard deviation. ****P < 0.0001. Statistical test: Mann-Whitney U