Table 4 Freeze-drying cycles used for MCP experiments
Phase | Shelf temp (°C) | Vacuum pressure set point (µBar) | Step hold time (HH:MM) |
|---|---|---|---|
Freezing | −50 | N/A | 04:00 |
Drying | −50 | 01 | 01:00–02:00 |
Drying | −40 | 01 | 01:00–02:00 |
Drying | −20 | 01 | 01:00–02:00 |
Drying | 0 | 01 | 01:00–02:00 |
Drying | 10 | 01 | 01:00–02:00 |
Drying | 20 | 01 | 01:00–02:00 |
Drying | 30 | 01 | 01:00–02:00 |
Drying | 40 | 01 | 01:00–02:00 |